![]() ![]() where the iso-electric point is 7 the pH should be lowered. When trying the latter option, shifting pH, the idea is to move away from the iso-electric point. When a peptide won't dissolve in pure water, and organic solvents like DMSO are not an option, then there are several things to try. Any buffer components that are required can then typically be added afterwards. Most peptides will dissolve in pure water. Once the peptide is in solution, it can be diluted further with pure water. If your application allows for it, then using an organic solvent like DMSO is a convenient way of dissolving your peptide. In practice, this peptide will dissolve properly at an elevated pH. For example, if we enter the sequence for the beta amyloid 1-40 peptide, the result will be "good water solubility". Determining the solubility of a peptide must be done by experiment. Please note that the result presented in this Peptide Calculator is only a very rough estimation of water solubility. Peptide Calculator uses the following values: The results depend on what index is used. Hydrophilic residues are shown as bars rising upwards, while hydrophobic have downwards bars. Innovagen's Peptide Calculator plots the hydrophathyįor each standard amino acid in the peptide sequence in a colour coded bar graph. This is repeated, using increments/decrements half the size of the previous, until the charge found equals 0, If the charge is > 0 the next pH to check is 7 + 3.5, if the charge is < 0 Innovagen's Peptide Calculatorįirst calculates the net charge for pH 7.0. The isoelectric point, pI, is the pH at which the net charge of the peptide is zero. ![]() Innovagen's Peptide Property Calculator uses the values taken from the CRC Handbook of Chemistry and Physics, 87th edition. The resulting net charge depends on what pKa values the algorithm uses.Only free terminii and the 20 naturally occuring amino acids and their D-forms are considered, all others are ignored.The residues are assumed to be independent of each other.This algorithm has its limitations, some of which are: Innovagen's Peptide Property Calculator calculates the netĬharge for all pH values of 0.1 to 14 in increments of 0.1, and plots these producing a titration curve. The j-index pertain to the C-terminus and the AsparticĪcid, Glutamic Acid, Cysteine, Tyrosine amino acids. The pKa values, of the N-terminus and the side chains of Arginine, Lysine,Īnd Histidine. ![]()
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